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L4025
Sigma
Lyticase from Arthrobacter luteus
lyophilized powder, ≥200 units/mg solid
| CAS Number: | 37340-57-1 |
| MDL number: | MFCD00131560 |
Description
| Biochem/physiol Actions | Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan. |
| Other Notes | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer |
| Unit Definition | One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture. |
| Application | Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species. |
Properties
| form | lyophilized powder |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 22 |
| WGK Germany | 3 |
References
| reference | Bir, N., et al. Prep. Biochem. 25, 171-181, (1995) |
| van Burik, J.A., et al. Med. Mycol 36, 299-303, (1998) | |
| Jazwinski, S.M. Meth. Enzymol. 182, 154-174, (1990) | |
| Phalip, V., et al. Biotechnol. Lett. 26, 409-413 , (2004) |
